Genotyping and primer details


Genotyping and primer details for SALK_029663 - At4g10040
Line ID SALK_029663
Locus At4g10040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:6278196 of line SALK_029663 Chr4:6278196
   
Insertion allele amplicon R204 - CE09
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CE09: 5'-TTATGCGCATCTCTTACGTATTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6278602
   
Insertion allele amplicon R212 - CE10
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer CE10: 5'-CCATATTTGTGAAATTGGACATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6277732
   
wt amplicon (over InsPos) CE09 - CE10
wt amplicon start Chr4:6277732
wt amplicon end Chr4:6278602
wt amplicon size 871 bp
Locus specific primer CE10: 5'-CCATATTTGTGAAATTGGACATCA-3'
2nd locus specific primer CE09: 5'-TTATGCGCATCTCTTACGTATTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11