Genotyping and primer details


Genotyping and primer details for SALK_029828c - At1g23030
Line ID SALK_029828c
Locus At1g23030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8156796 of line SALK_029828c Chr1:8156796
   
Insertion allele amplicon LBb3 - 08ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 08ii: 5'-TCTCAAGCTTCCATGTAACACATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8157257
   
wt amplicon (over InsPos) 12ir - 08ii
wt amplicon start Chr1:8156555
wt amplicon end Chr1:8157257
wt amplicon size 703 bp
Locus specific primer 08ii: 5'-TCTCAAGCTTCCATGTAACACATT-3'
2nd locus specific primer 12ir: 5'-GACCAAGACGTAGGTCCTTAAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11