Genotyping and primer details


Genotyping and primer details for SALK_029945c - At2g32010
Line ID SALK_029945c
Locus At2g32010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13627217 of line SALK_029945c Chr2:13627217
   
Insertion allele amplicon LBb3 - 42kq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 42kq: 5'-CTATTCTCGGCCTAGTGATGTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13626753
   
wt amplicon (over InsPos) 02mu - 42kq
wt amplicon start Chr2:13626753
wt amplicon end Chr2:13627515
wt amplicon size 763 bp
Locus specific primer 42kq: 5'-CTATTCTCGGCCTAGTGATGTTTC-3'
2nd locus specific primer 02mu: 5'-CTCTAGTAATGCCCTCCAGTTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11