Sequences from confirmation amplicon


Confirmation sequences for line SALK_029945c and hit At2g32010 are shown.
Line ID SALK_029945c
Locus At2g32010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13627217
Primers used for PCR 42kq - LBb3, show primer details
   
Sequence Name 03-K114130-SALK_029945C-R210
GenBank Accession LN484344 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114130-SALK_029945C-R210
TTAGCTCATCACCTTCTTTTTGTCCCGAGGTCAAATGGGTGCACACAAAGCAGAAGCTTG
TTTGATGAAGCAACATACTAATTGAGATTGATCCCTACAAGAACCACAGAAGCACATTAT
AGCAATGCACTAACTAACCAACTAAGTCAAGCGGTAACAAACATAAGATTTTTTACCTTA
TTTCCGAGATAACCCATTAATCCTCTACCAACACAAGATACTTTCATGTTCTTGACGTGT
TCTCGTAACTCGCTTTTCACCCAAATGGTTAAAAAGATACCAACCATCTGTTTACTTGCG
ACCAAACAGTACTGCGATGAGTTCCACGGGGTTCTGTAACCGTTTTCGTTTGCTGCAGAA
CCAAGAAATGACCCCGGTGAATTCAAAAATGTGCTTGGAGAATCCCCTGGGCCGTTATCA
TCATCCGAGGAGCCCCACCTGGAAACATCACTAGGCGAAGAATA
   
Sequence Name 13-K114131-SALK_029945C-42kq
GenBank Accession LN484644 [GenBank]
Sequencing primer gene specific primer, 42kq: 5'-CTATTCTCGGCCTAGTGATGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K114131-SALK_029945C-42kq
CGGCCAGGGGATTCTCCAAGCACATTTTTGAATTCCCGGGGTCATTTCTTGGTTCTGCAG
CAAACGAAAACGGTTACAGAACCCCGTGGAACTCATCGCAGTACTGTTTGGTCGCAAGTA
AACAGATGGTTGGTATCTTTTTAACCATTTGGGTGAAAAGCGAGTTACGAGAACACGTCA
AGAACATGAAAGTATCTTGTGTTGGTAGAGGATTAATGGGTTATCTCGGAAATAAGGTAA
AAAATCTTATGTTTGTTACCGCTTGACTTAGTTGGTTAGTTAGTGCATTGCTATAATGTG
CTTCTGTGGTTCTTGTAGGGATCAATCTCAATTAGTATGTTGCTTCATCAAACAAGCTTC
TGCTTTGTGTGCACCCATTTGACCTCGGGACAAAAAGAAGGTGATGAGCTAAGGAGAAAC
AAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGT
TTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGA
GAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGT
GGTTCCG

Last Updated on 06.12.2018 16:11