Genotyping and primer details


Genotyping and primer details for SALK_030651c - At5g19380
Line ID SALK_030651c
Locus At5g19380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6526842 of line SALK_030651c Chr5:6526842
   
Insertion allele amplicon LBb3 - 18ma
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18ma: 5'-TGCTTTCATGGAAACCTACTTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6527417
   
wt amplicon (over InsPos) 02ng - 18ma
wt amplicon start Chr5:6526614
wt amplicon end Chr5:6527417
wt amplicon size 804 bp
Locus specific primer 18ma: 5'-TGCTTTCATGGAAACCTACTTCTT-3'
2nd locus specific primer 02ng: 5'-CGGTCACTCTAAACATTATGGGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11