Genotyping and primer details


Genotyping and primer details for SALK_031043 - At4g16265
Line ID SALK_031043
Locus At4g16265 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9203735 of line SALK_031043 Chr4:9203735
   
Insertion allele amplicon LBb3 - 29eq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29eq: 5'-TTTTTAAAAGGTCTTGCGTTTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9203250
   
wt amplicon (over InsPos) 21gc - 29eq
wt amplicon start Chr4:9203250
wt amplicon end Chr4:9204002
wt amplicon size 753 bp
Locus specific primer 29eq: 5'-TTTTTAAAAGGTCTTGCGTTTGTT-3'
2nd locus specific primer 21gc: 5'-AGCTCGTTATCTAAAGATTCTGCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11