Sequences from confirmation amplicon


Confirmation sequences for line SALK_031043 and hit At4g16265 are shown.
Line ID SALK_031043
Locus At4g16265 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9203735
Primers used for PCR 29eq - LBb3, show primer details
   
Sequence Name 26-K111273-SALK_031043C-29eq
GenBank Accession LN485030 [GenBank]
Sequencing primer gene specific primer, 29eq: 5'-TTTTTAAAAGGTCTTGCGTTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K111273-SALK_031043C-29eq
ATAGCACACTATCTTAACCATCAGATTCATGATATGAGAGACAAGGAAAATGATCTAAAC
TCATTAAATTATCATTCCTTGGTTTACTTCAAGTTCAGACATTCGACCTTTAACCGTATA
AGCGATTACAAAGAGTAAAACATCAGAGCTTAAATAAATATCAACACAAATCATATACAA
GTGAACAACTAAAGTCTATTGGTTTCAGCCCTAGCTTTGTTTCAACACCACATTGTTCCA
TTAACACTAAGGAAACCTAATTTTATAAAAGAAAGCATAGTAAACAAAGTTTACCTGGTG
ATCACAATTACGGCACGCATAGAGGAGGATTGATTGCTCCTTGTCTTCCTTGGGATATAG
AATGTTATTACTGAACATTTCAAAAAAAAACAAAATCAAAGGTTCATAAGCTGCAAAAGT
GGAGAAAAGTTGGAGCTTTAATCCACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTT
TCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAG
TTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT
TCC
   
Sequence Name 10-K111272-SALK_031043C-R210
GenBank Accession LN484547 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K111272-SALK_031043C-R210
ACTTTTCTCCTTTTGCAGCTAGAACCTTTGATTTTGTTTTTTTTTGAAATGTTCAGTAAT
AACATTCTATATCCCAAGGAAGACAAGGAGCAATCAATCCTCCTCTATGCGTGCCGTAAT
TGTGATCACCAGGTAAACTTTGTTTACTATGCTTTCTTTTATAAAATTAGGTTTCCTTAG
TGTTAATGGAACAATGTGGTGTTGAAACAAAGCTAGGGCTGAAACCAATAGACTTTAGTT
GTTCACTTGTATATGATTTGTGTTGATATTTATTTAAGCTCTGATGTTTTACTCTTTGTA
ATCGCTTATACGGTTAAAGGTCGAATGTCTGAACTTGAAGTAAACCAAGGAATGATAATT
TAATGAGTTTAGATCATTTTCCTTGTCTCTCATATCATGAATCTGATTGGTTAAGATAGT
AGTGCTTATCAGTATAAAGAGTTCTTTGAGAACTTCTGAACAAACGCAAGACTTTTTTAA
AA

Last Updated on 06.12.2018 16:11