Genotyping and primer details


Genotyping and primer details for SALK_031682c - At5g20580
Line ID SALK_031682c
Locus At5g20580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6958807 of line SALK_031682c Chr5:6958807
   
Insertion allele amplicon LBb3 - 49dl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 49dl: 5'-AAATGAAAATTGTGTGTCTTGCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6959396
   
wt amplicon (over InsPos) 04ee - 49dl
wt amplicon start Chr5:6958253
wt amplicon end Chr5:6959396
wt amplicon size 1144 bp
Locus specific primer 49dl: 5'-AAATGAAAATTGTGTGTCTTGCAG-3'
2nd locus specific primer 04ee: 5'-TAGAAAAGCCGGAGAAAAATATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11