Genotyping and primer details


Genotyping and primer details for SALK_032042 - At1g17560
Line ID SALK_032042
Locus At1g17560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6037991 of line SALK_032042 Chr1:6037991
   
Insertion allele amplicon LBb3 - 23kn
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23kn: 5'-CATTCACTCTCTGCGACACTAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6038621
   
wt amplicon (over InsPos) 08lz - 23kn
wt amplicon start Chr1:6037564
wt amplicon end Chr1:6038621
wt amplicon size 1058 bp
Locus specific primer 23kn: 5'-CATTCACTCTCTGCGACACTAAAG-3'
2nd locus specific primer 08lz: 5'-CAAGTGATGAATGGATCAAAGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11