Genotyping and primer details


Genotyping and primer details for SALK_032139 - At1g14290
Line ID SALK_032139
Locus At1g14290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4881763 of line SALK_032139 Chr1:4881763
   
Insertion allele amplicon LBb3 - 07bh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07bh: 5'-ACATTAAAATTGAAACCAGTTGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4882083
   
wt amplicon (over InsPos) 03da - 07bh
wt amplicon start Chr1:4881393
wt amplicon end Chr1:4882083
wt amplicon size 691 bp
Locus specific primer 07bh: 5'-ACATTAAAATTGAAACCAGTTGGC-3'
2nd locus specific primer 03da: 5'-CCCTAATTCATTCTCATCGACCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11