Genotyping and primer details


Genotyping and primer details for SALK_032851c - At3g21465
Line ID SALK_032851c
Locus At3g21465 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7562904 of line SALK_032851c Chr3:7562904
   
Insertion allele amplicon LBb3 - 28jr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28jr: 5'-CCTAACGATAAATGTTCCTCCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7563531
   
wt amplicon (over InsPos) 11ll - 28jr
wt amplicon start Chr3:7562300
wt amplicon end Chr3:7563531
wt amplicon size 1232 bp
Locus specific primer 28jr: 5'-CCTAACGATAAATGTTCCTCCAAA-3'
2nd locus specific primer 11ll: 5'-CAGATCATTTTTGGTAGCCAATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11