Genotyping and primer details


Genotyping and primer details for SALK_033186c - At1g14240
Line ID SALK_033186c
Locus At1g14240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4865184 of line SALK_033186c Chr1:4865184
   
Insertion allele amplicon LBb3 - 27dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27dh: 5'-TTTCCCAACTTATCCAAAATGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4865785
   
wt amplicon (over InsPos) 97dy - 27dh
wt amplicon start Chr1:4864578
wt amplicon end Chr1:4865785
wt amplicon size 1208 bp
Locus specific primer 27dh: 5'-TTTCCCAACTTATCCAAAATGAAG-3'
2nd locus specific primer 97dy: 5'-AGATGGTTTTGTGATATTTGGCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11