Genotyping and primer details


Genotyping and primer details for SALK_033970c - At1g14350
Line ID SALK_033970c
Locus At1g14350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4910336 of line SALK_033970c Chr1:4910336
   
Insertion allele amplicon LBb3 - 53us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 53us: 5'-ACACTGGTGCCTTCTTTTGTAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4910965
   
wt amplicon (over InsPos) 30vl - 53us
wt amplicon start Chr1:4909883
wt amplicon end Chr1:4910965
wt amplicon size 1083 bp
Locus specific primer 53us: 5'-ACACTGGTGCCTTCTTTTGTAGAT-3'
2nd locus specific primer 30vl: 5'-CAAACTTCGCAGCATATCAAAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11