Genotyping and primer details


Genotyping and primer details for SALK_034210 - At2g33320
Line ID SALK_034210
Locus At2g33320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14119632 of line SALK_034210 Chr2:14119632
   
Insertion allele amplicon LBb3 - 39dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39dr: 5'-CAACTCCGAGAAGATCTAACATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14120154
   
wt amplicon (over InsPos) 06hi - 39dr
wt amplicon start Chr2:14119178
wt amplicon end Chr2:14120154
wt amplicon size 977 bp
Locus specific primer 39dr: 5'-CAACTCCGAGAAGATCTAACATCC-3'
2nd locus specific primer 06hi: 5'-TTAAAATTCTGAACCGAATCCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11