Genotyping and primer details


Genotyping and primer details for SALK_034354c - At2g32060
Line ID SALK_034354c
Locus At2g32060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13639253 of line SALK_034354c Chr2:13639253
   
Insertion allele amplicon LBb3 - 32cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 32cv: 5'-GGAGTTGACTGTAAGGAAATCTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13639778
   
wt amplicon (over InsPos) 98dy - 32cv
wt amplicon start Chr2:13638835
wt amplicon end Chr2:13639778
wt amplicon size 944 bp
Locus specific primer 32cv: 5'-GGAGTTGACTGTAAGGAAATCTCG-3'
2nd locus specific primer 98dy: 5'-CGTTCTTTCTTTCTTTTTCCCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11