Sequences from confirmation amplicon


Confirmation sequences for line SALK_034354c and hit At2g32060 are shown.
Line ID SALK_034354c
Locus At2g32060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13639253
Primers used for PCR 32cv - LBb3, show primer details
   
Sequence Name 19-K109252-SALK_034354C-32cv
GenBank Accession LN484808 [GenBank]
Sequencing primer gene specific primer, 32cv: 5'-GGAGTTGACTGTAAGGAAATCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K109252-SALK_034354C-32cv
GCGCTAGCTTATTGAGAAGCGTAATGCTCAGCTCTGTGTCCTGGCTGAAGATTGCAACCA
ACCTGATTATGTCAAGCTTGTTAAAGCTCTTTGCGCTGACCACAGCATCAAATTGCTTAC
TGTTCCAAGTGCCAAAACACTCGGCGAATGGGCTGGTGTAAGTTGTCTTCCTTTTTTATC
CTTGAAGCTTTCTCTCCTCTAAGGAAGACTTTGATGGTAACTAATAATCTTCAATTTGGT
TGCAGCTTTGCAAGATTGATTCTGAGGGCAATGCAAGGAAGGTTGTTGGATGCTCATGTC
TCGTGATCAAGGTAACAAATCCTTTTTACATTCTCTTAATCCATGAATCATAGGAACCTC
TAGATACTTGCAAAGTCATTGAGAAATGTGCCTGGTGGTTTATTTGTGTGGTTCTGTGTT
GATTGTTACAGGATTTCGGTGAGGAGACAACTGCACTAAACATTGTGGACGACAATCTGA
TCATGATATATTGATAGTTTATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 29-K109255-SALK_034354C-R210
GenBank Accession LN485101 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K109255-SALK_034354C-R210
GTCATTTGTTTACCCACAATATAAACTATCAATATATCATGATCAGATTGTCGTCCACAA
TGTTTAGTGCAGTTGTCTCCTCACCGAAATCCTGTAACAATCAACACAGAACCACACAAA
TAAACCACCAGGCACATTTCTCAATGACTTTGCAAGTATCTAGAGGTTCCTATGATTCAT
GGATTAAGAGAATGTAAAAAGGATTTGTTACCTTGATCACGAGACATGAGCATCCAACAA
CCTTCCTTGCATTGCCCTCAGAATCAATCTTGCAAAGCTGCAACCAAATTGAAGATTATT
AGTTACCATCAAAGTCTTCCTTAGAGGAGAGAAAGCTTCAAGGATAAAAAAGGAAGACAA
CTTACACCAGCCCATTCGCCGAGTGTTTTGGCACTTGGAACAGTAAGCAATTTGATGCTG
TGGTCAGCGCAAAGAGCTTTAACAAGCTTGACATAATCAGGTTGGTTGCAATCTTCAGCC
AGGACACAGAGCTGAGCATTACGCTTCTCAATAAGCTTAGCGCTTTCATGGAGACCACGA
ACAACACCACCATAAGCACGAGATTTCCTTACAGTCAACT

Last Updated on 06.12.2018 16:11