Genotyping and primer details

Genotyping and primer details for SALK_035225c - At5g60160
Line ID SALK_035225c
Locus At5g60160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24225145 of line SALK_035225c Chr5:24225145
Insertion allele amplicon LBb3 - CG51
Locus specific primer CG51: 5'-CCACTATTTGAGATCAGAACCCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24225693
wt amplicon (over InsPos) CG52 - CG51
wt amplicon start Chr5:24224745
wt amplicon end Chr5:24225693
wt amplicon size 949 bp
Locus specific primer CG51: 5'-CCACTATTTGAGATCAGAACCCAT-3'
2nd locus specific primer CG52: 5'-TCCAGATGACGTTGCATCTATAAG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11