Sequences from confirmation amplicon


Confirmation sequences for line SALK_035225c and hit At5g60160 are shown.
Line ID SALK_035225c
Locus At5g60160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24225145
Primers used for PCR CG51 - LBb3, show primer details
   
Sequence Name 44-K110394-SALK_035225C-CG51
GenBank Accession LN485371 [GenBank]
Sequencing primer gene specific primer, CG51: 5'-CCACTATTTGAGATCAGAACCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K110394-SALK_035225C-CG51
GCAGATTATCTATGAAGTCGAACCATTGTTGTCAAGTTTGTAGCAGACTTGAATTCTTAC
TTCCTTTGCAATTTATTTATATTGTGTGATATATTTGAAGGCGGAGCTCAATAAAACGCC
AGCTGAAAGTGGTGAACATGACGAAGGAAAGAAGTGTGCTGAAACTAGTTCTAAATCAAA
GCATCATCCGCTTCTAATGGAGGTCCAGTTGAAATATATAAGCTTTATGGTTTTAGCTTG
AATTTTAGATCTTGTTCTGGTTTAGTAACCAAGTAGTCCTCTTTTTAATTTCTTAGATCA
TTGCAAACGCACTGGGTTGTAAACCTGAGGAGATATGCGACTTTGAGCTGCAAGCATGTG
ACACTCAGCCAAGTATACTAGCTGGTGCAGCTAAAGAATTTATTTTCTCGGGAAGACTAG
ATAATCTCTGCATGTCATTTTGCTCTCTGAAGGTACGTCCTTTACTTTTACTTTTAGAGG
AGAAAATAGTCCATGGTCTATTTAATGTATTGTGGTGTAACACATTGCGGACGTTTTTAA
TGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACC
GCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAA
TCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 36-K110393-SALK_035225C-R210
GenBank Accession LN485233 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K110393-SALK_035225C-R210
TAGACCATGGACTATTTTCTCCTCTAAAGTAAAAGTAAAGGACGTACCTTCAGAGAGCAA
AATGACATGCAGAGATTATCTAGTCTTCCCGAGAAAATAAATTCTTTAGCTGCACCAGCT
AGTATACTTGGCTGAGTGTCACATGCTTGCAGCTCAAAGTCGCATATCTCCTCAGGTTTA
CAACCCAGTGCGTTTGCAATGATCTAAGAAATTAAAAAGAGGACTACTTGGTTACTAAAC
CAGAACAAGATCTAAAATTCAAGCTAAAACCATAAAGCTTATATATTTCAACTGGACCTC
CATTAGAAGCGGATGATGCTTTGATTTAGAACTAGTTTCAGCACACTTCTTTCCTTCGTC
ATGTTCACCACTTTCAGCTGGCGTTTTATTGAGCTCCGCCTTCAAATATATCACACAATA
TAAATAAATTGCAAAGGAAGTAAGAATTCAAGTCTGCTACAAACTTGACAACAATGGTTT
CGACTTCATAGATAAATCTGCCAAAAGTTAAGGGGTTCACAGAGATGGGTTCTGATCTCA
AATAGTGG

Last Updated on 06.12.2018 16:11