Genotyping and primer details


Genotyping and primer details for SALK_035250c - At1g72690
Line ID SALK_035250c
Locus At1g72690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27361415 of line SALK_035250c Chr1:27361415
   
Insertion allele amplicon LBb3 - 16td
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16td: 5'-AGTCTTCTTCTTCTTCGCTTCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27360943
   
wt amplicon (over InsPos) 03ue - 16td
wt amplicon start Chr1:27360943
wt amplicon end Chr1:27361907
wt amplicon size 965 bp
Locus specific primer 16td: 5'-AGTCTTCTTCTTCTTCGCTTCCTT-3'
2nd locus specific primer 03ue: 5'-CTGGTAATCGCTGTTAAAGACTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11