Sequences from confirmation amplicon


Confirmation sequences for line SALK_035250c and hit At1g72690 are shown.
Line ID SALK_035250c
Locus At1g72690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27361415
Primers used for PCR 16td - LBb3, show primer details
   
Sequence Name 20-K116922-SALK_035250C-16td
GenBank Accession LN484862 [GenBank]
Sequencing primer gene specific primer, 16td: 5'-AGTCTTCTTCTTCTTCGCTTCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116922-SALK_035250C-16td
ATCATTTTGCTTTTGCAGTGATTCAATTTCAATCTGGTCTGGTGTTTTTTTTCCTTGGAC
TCACTTACAAACAAAAAGGCCCATTATAACCCAACAAGGCCCATTTAAGTTGAAATCGAT
TTTGATTTATACTGTAAAGACTCTTTTGCATCTGACGCAAAACGCCACCGAATCAGGAAC
CAAAAATAGAAAGCTGCAAGATCAAGAAGACAAAAACCATCGATGGCGGATCAAAACCGA
TCAGGTAAACACAATGAACGATCAGATCTCATCATAGGTTTATGCATATTGTTAAAAAGG
AAGACTTTTTGATCAAGTTATGTTCGTTTCCTCAAATTTCACAATCAAAATTACACGTCT
ATTAATGGATGGTTTTGATCGGAAATTTCTCTAAAGAAACAATAGAAACACGCCAAATGA
CACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGT
GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTG
AGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAT
GGTGGTTCCG
   
Sequence Name 09-K116921-SALK_035250C-R210
GenBank Accession LN484530 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K116921-SALK_035250C-R210
TCATTTGGCGTGTTTCTATTGTTTCTTTAGAGAAATTTCCGATCAAAACCATCCATTAAT
AGACGTGTAATTTTGATTGTGAAATTTGAGGAAACGAACATAACTTGATCAAAAAGTCTT
CCTTTTTAACAATATGCATAAACCTATGATGAGATCTGATCGTTCATTGTGTTTACCTGA
TCGGTTTTGATCCGCCATCGATGGTTTTTGTCTTCTTGATCTTGCAGCTTTCTATTTTTG
GTTCCTGATTCGGTGGCGTTTTGCGTCAGATGCAAAAGAGTCTTTACAGTATAAATCAAA
ATCGATTTCAACTTAAATGGGCCTTGTTGGGTTATAATGGGCCTTTTTGTTTGTAAGTGA
GTCCAAGGAAAAAAAACACCAGACCAGATTGAAATTGAATCACTTGCAAAAGCAAAATTG
ATTACTCTGTTTTGGTTTTGGATCAGATCAACCAAAGGAAGCGAAGAGATAGGAAGACT

Last Updated on 06.12.2018 16:11