Genotyping and primer details


Genotyping and primer details for SALK_035827c - At5g52240
Line ID SALK_035827c
Locus At5g52240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21212911 of line SALK_035827c Chr5:21212911
   
Insertion allele amplicon LBb3 - 19ay
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19ay: 5'-TTTTTGGGAAACATTTAAGACTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21212146
   
wt amplicon (over InsPos) 11cd - 19ay
wt amplicon start Chr5:21212146
wt amplicon end Chr5:21213180
wt amplicon size 1035 bp
Locus specific primer 19ay: 5'-TTTTTGGGAAACATTTAAGACTTTG-3'
2nd locus specific primer 11cd: 5'-AGAAAGACCTGTGTAAGCATGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11