Genotyping and primer details


Genotyping and primer details for SALK_036476c - At3g13730
Line ID SALK_036476c
Locus At3g13730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4498680 of line SALK_036476c Chr3:4498680
   
Insertion allele amplicon LBb3 - 72hw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 72hw: 5'-TAAGCTGATATTACCTCGTGGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4499289
   
wt amplicon (over InsPos) 06mc - 72hw
wt amplicon start Chr3:4498113
wt amplicon end Chr3:4499289
wt amplicon size 1177 bp
Locus specific primer 72hw: 5'-TAAGCTGATATTACCTCGTGGCTT-3'
2nd locus specific primer 06mc: 5'-GGAATACGACAAAATAGTTGGCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11