Sequences from confirmation amplicon


Confirmation sequences for line SALK_036476c and hit At3g13730 are shown.
Line ID SALK_036476c
Locus At3g13730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4498680
Primers used for PCR 72hw - LBb3, show primer details
   
Sequence Name 04-K113932-SALK_036476C-72hw
GenBank Accession LN484373 [GenBank]
Sequencing primer gene specific primer, 72hw: 5'-TAAGCTGATATTACCTCGTGGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K113932-SALK_036476C-72hw
AGCTATCTACTTTATGAATTTCTTTTTTTTTCATTAGGAAGAAAACATGAAGCTGAAAAG
TTTGAAGGAATTGACAGGAGAGCCACTATATTGGAATGACTACTTGTCGTTACCTTTTAC
ACAAAAGGTATATAATTTCTTCTTTTCCCTATTCATTAGTTAATACTCAACAAGGTGATT
AATTAAATTTTTAATTATCATCAGGTGATTACAGAGACACTGAGAATGGGAAATGTTATA
ATTGGAGTGATGAGAAAGGCGATGAAAGATGTTGAAATAAAAGGATATGTGATACCAAAA
GGATGGTGTTTCTTGGCCTATCTCAGATCAGTTCATCTTGATAAGCTTTATTATGAGTCT
CCCTACAAATTTAATCCCTGGAGATGGCAAGTACGTAAGATCACCAAAATATATTTAACA
TATAATCTTTTACTTAATACGAATTATATATTTAATTCATTTCTTCACAAAATAGGAAAG
GGACATGAACACGAGTAGTTTCAGTCCTTTTGGAGGTGGTCAGAGATTGTGCCCTGGTCT
CGATTTGGCTCGTCTTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTG
CGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTG
ATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCC
CAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 02-K113931-SALK_036476C-R210
GenBank Accession LN484312 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K113931-SALK_036476C-R210
CATTTGTTTACCCCAAGACGAGCCAAATCGAGACCAGGGCACAATCTCTGACCACCTCCA
AAAGGACTGAAACTACTCGTGTTCATGTCCCTTTCCTATTTTGTGAAGAAATGAATTAAA
TATATAATTCGTATTAAGTAAAAGATTATATGTTAAATATATTTTGGTGATCTTACGTAC
TTGCCATCTCCAGGGATTAAATTTGTAGGGAGACTCATAATAAAGCTTATCAAGATGAAC
TGATCTGAGATAGGCCAAGAAACACCATCCTTTTGGTATCACATATCCTTTTATTTCAAC
ATCTTTCATCGCCTTTCTCATCACTCCAATTATAACATTTCCCATTCTCAGTGTCTCTGT
AATCACCTGATGATAATTAAAAATTTAATTAATCACCTTGTTGAGTATTAACTAATGAAT
AGGGAAAAGAAGAAATTATATACCTTTTGTGTAAAAGGTAACGACAAGTAGTCATTCCAA
TATAGTGGCTCTCCTGTCAATTCCTTCAAACTTTTCAGCTTCATGTTTTCTTCCTAATGA
AAAAAAAAGAAATTCATAAAGTAAGATTAGCTTATAGATTTTTAGTGGCCAATCATATAA
AGCCACGAGGTATATTCAGCT

Last Updated on 06.12.2018 16:11