Genotyping and primer details


Genotyping and primer details for SALK_036965c - At4g26430
Line ID SALK_036965c
Locus At4g26430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13355884 of line SALK_036965c Chr4:13355884
   
Insertion allele amplicon LBb3 - 37fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37fx: 5'-TGATTAACAAATTGAAGAGGGACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13356438
   
wt amplicon (over InsPos) 02ho - 37fx
wt amplicon start Chr4:13355556
wt amplicon end Chr4:13356438
wt amplicon size 883 bp
Locus specific primer 37fx: 5'-TGATTAACAAATTGAAGAGGGACC-3'
2nd locus specific primer 02ho: 5'-TTGTCGATTGTTTGATACTCTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11