Genotyping and primer details


Genotyping and primer details for SALK_037273c - At1g04750
Line ID SALK_037273c
Locus At1g04750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1333308 of line SALK_037273c Chr1:1333308
   
Insertion allele amplicon R204 - AW09
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW09: 5'-CCACCATATCGCTTGTTAAAATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1332637
   
wt amplicon (over InsPos) 05lt - AW09
wt amplicon start Chr1:1332637
wt amplicon end Chr1:1333469
wt amplicon size 833 bp
Locus specific primer AW09: 5'-CCACCATATCGCTTGTTAAAATCT-3'
2nd locus specific primer 05lt: 5'-GATCAGACGGTCGATTGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11