Genotyping and primer details


Genotyping and primer details for SALK_038310c - At3g02800
Line ID SALK_038310c
Locus At3g02800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:607559 of line SALK_038310c Chr3:607559
   
Insertion allele amplicon R204 - SC07
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer SC07: 5'-GGTCAAATAAAAGCAGAGAAAACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:607993
   
wt amplicon (over InsPos) 01ah - SC07
wt amplicon start Chr3:607217
wt amplicon end Chr3:607993
wt amplicon size 777 bp
Locus specific primer SC07: 5'-GGTCAAATAAAAGCAGAGAAAACG-3'
2nd locus specific primer 01ah: 5'-CCAACTAAAACTTTGAGAGCATCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11