Genotyping and primer details


Genotyping and primer details for SALK_038326c - At3g14290
Line ID SALK_038326c
Locus At3g14290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4766299 of line SALK_038326c Chr3:4766299
   
Insertion allele amplicon LBb3 - 53cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 53cs: 5'-GGTTAAATTCAGTGAGGGTGGTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4766718
   
wt amplicon (over InsPos) 31dw - 53cs
wt amplicon start Chr3:4765747
wt amplicon end Chr3:4766718
wt amplicon size 972 bp
Locus specific primer 53cs: 5'-GGTTAAATTCAGTGAGGGTGGTTA-3'
2nd locus specific primer 31dw: 5'-ACTTATGCTCGGTCTCACAAACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11