Sequences from confirmation amplicon


Confirmation sequences for line SALK_038326c and hit At3g14290 are shown.
Line ID SALK_038326c
Locus At3g14290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4766299
Primers used for PCR 53cs - LBb3, show primer details
   
Sequence Name 85-K109033-SALK_038326C-53cs
GenBank Accession LN486283 [GenBank]
Sequencing primer gene specific primer, 53cs: 5'-GGTTAAATTCAGTGAGGGTGGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K109033-SALK_038326C-53cs
CTATATTGAGATTTTTTTTCCGACGTACATGATGATATGTACATAATAGATCTCTAATTG
CAGTTCTGATTTTTTCTTTTGGACAGATATTTACTATTTTTAACTTCTTGTTGAAAACGA
CAAAAAAATGTAGATAGAAAGCTCAATAAAACTCAACTCGATAAAATCCATTATCTGATT
AAACGAAACTAATTTTTGGTTTAGAGAACCGGAAGCAGACAGAAGCCTGATAAATTACCG
AACAGACAAGGTAAGATAACAGGAAGAGAATGGCGGTTTATTTCTTCATAGACGACTGAT
GACGGCTTCAACCTCCTGAGGAGTGTAGAGGTGATAAGCTGGTGCGACTTTGGCAATGTC
GACATTGTGGTGTAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTT
AATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAA
AATCCTGTTTGATGGTGGTTCCGAAATCGACAAAA
   
Sequence Name 69-K109032-SALK_038326C-R210
GenBank Accession LN485972 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K109032-SALK_038326C-R210
GTCATTTGTACACCACAATGTCGACATTGCCAAAGTCGCACCAGCTTATCACCTCCACAC
TCCTCAGGAGGTTGAAGCCGTCATCAGTCGTCTATGAAGAAATAAACCGCCATTCTCTTC
CTGTTATCTTACCTTGTCTGTTCGGTAATTTATCAGGCTTCTGTCTGCTTCCGGTTCTCT
AAACCAAAAATTAGTTTCGTTTAATCAGATAATGGATTTTATCGAGTTGAGTTTTATTGA
GCTTTCTATCTACATTTTTTTGTCGTTTTCAACAAGAAGTTAAAACTAATAAATATCTGT
CCAAAAGAAAAAATCAGAACTTGAAAGTTGAAATCTATTATGTACATATCATCATTGTAC
GTCGGAAAAAAAATCTCAATTATAAGATAATTATATATTTATTATCTATATAACCACCCT
CACTGAATTTAAC

Last Updated on 06.12.2018 16:11