Genotyping and primer details


Genotyping and primer details for SALK_038477c - At3g59990
Line ID SALK_038477c
Locus At3g59990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22156317 of line SALK_038477c Chr3:22156317
   
Insertion allele amplicon LBb3 - 58fr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 58fr: 5'-TTACGCTGCTGATGGTTATATACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22155383
   
wt amplicon (over InsPos) 01jw - 58fr
wt amplicon start Chr3:22155383
wt amplicon end Chr3:22156603
wt amplicon size 1221 bp
Locus specific primer 58fr: 5'-TTACGCTGCTGATGGTTATATACG-3'
2nd locus specific primer 01jw: 5'-CATGTTATGTCCTTTTGGCAGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11