Genotyping and primer details


Genotyping and primer details for SALK_039424c - At3g06170
Line ID SALK_039424c
Locus At3g06170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1867783 of line SALK_039424c Chr3:1867783
   
Insertion allele amplicon LBb3 - 76by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 76by: 5'-AAACAATGTCTGTTTTGAGACGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1866784
   
wt amplicon (over InsPos) 45lt - 76by
wt amplicon start Chr3:1866784
wt amplicon end Chr3:1868086
wt amplicon size 1303 bp
Locus specific primer 76by: 5'-AAACAATGTCTGTTTTGAGACGTG-3'
2nd locus specific primer 45lt: 5'-TTTTGCAACACTCAAAATTCTCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11