Sequences from confirmation amplicon


Confirmation sequences for line SALK_039424c and hit At3g06170 are shown.
Line ID SALK_039424c
Locus At3g06170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1867783
Primers used for PCR 76by - LBb3, show primer details
   
Sequence Name 33-K108288-SALK_039424C-R210
GenBank Accession LN485182 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K108288-SALK_039424C-R210
ATTTGTTACCCACAATATATCTCTTTCATTCCATCATCAATCTCGGCTAAAAGGAACCTC
CTTTTTCACAAACCCAGTTCAGAAAAGGATGAGAGAATCGTACAAGGGAGTTTCTCGAGG
AGCGGAGCACCAGTCTCACGAAGAATCCAGGAGACGACAAGAGAGGCACCGAAGAGACCA
CAATAAGCGATCCTCGCTGATTTCCTCGATATCCCCGATGCTACCGACGAGCATAATCCA
CAAGTCGCCGACGCACAGCACGAAGCTAAGCAACACGCCATTGTTTCAGAGATTTTTTTT
TGTTTTGACTTTCACAATAATCTGTTCTTCTGTTGTGAAGAAGAGATATCACATTTTCGA
GAAATTACGAAATCTGTACCTAACATATTAAAACGATCATTCTAACCCATTTTACATGAT
TGTAACTTGTAAGAGAAACAGAGTGATTAACGGGTAAGCTTAAGCCTGCTCCTCATTGTG
TTTACTTCAAAGTCTACAAGAGAACTCAAAAGTCTTAAACATGTTTTGAAATTAGACATT
CTAATAGTTTACTACAAAAGACTAGACTCGAAAGAGAAGAAACACAATTGCAGAACTGCA
TCAGAGAATCAAGTGCTTGCTGCTGCATCGCGGAAGATGTGAATCTTGATTGCATAAACC
ACTTTGTCCTCGTTGCGGAGAATTTCACATAGGATGGAGTCATTCTCTGTGAACTGGTTT
CTCTCGCATATTTTCTGCCATTTGTTTATGCAAACCCGTTGATCTGCCCACTTGGTCGTT
TTCGCGGTTAGTTTTCCATATCCGTCAATATAGTCAACATTGTCGCTGAATATCAAGCCG
TAGTCTTTCACCAACTGTGCATGAACTAACTGTGCATAATGTATAATAGAGCTTTAGTTA
GAAAATAATCATCAACTCGTGAAAGGATTTTTAATGAGACGAGATAATAATA
   
Sequence Name 35-K108289-SALK_039424C-76by
GenBank Accession LN485215 [GenBank]
Sequencing primer gene specific primer, 76by: 5'-AAACAATGTCTGTTTTGAGACGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K108289-SALK_039424C-76by
AGTTGAGCGGTAGACTTATTAGATATTATTATCTCGTCTCATTAAAATCCTTTCACGAGT
TGATGATTATTTTTCTAACTAAAGCTCTATATACATTATGCACAGTTAGTTCATGCACAG
TTGGTGAAAGACTACGGCTTGATATTCAGCGACAATGTTGACTATATTGACGGATATGGA
AAACTAACCGCGAAAACGACCAAGTGGGCAGATCAACGGGTTTGCATAAACAAATGGCAG
AAAATATGCGAGAGAAACCAGTTCACAGAGAATGACTCCATCCTATGTGAAATTCTCCGC
AACGAGGACAAAGTGGTTTATGCAATCAAGATTCACATCTTCCGCGATGCAGCAGCAAGC
ACTTGATTCTCTGATGCAGTTCTGCAATTGTGTTTCTTCTCTTTCGAGTCTAGTCTTTTG
TAGTAAACTATTAGAATGTCTAATTTCAAAACATGTTTAAGACTTTTGAGTTCTCTTGTA
GACTTTGAAGTAAACACAATGAGGAGCAGGCTTAAGCTTACCCGTTAATCACTCTGTTTC
TCTTACAAGTTACAATCATGTAAAATGGGTTAGAATGATCGTTTTAATATGTTAGGTACA
GATTTCGTAATTTCTCGAAAATGTGATATCTCTTCTTCACAACAGAAGAACAGATTATTG
TGAAAGTCAAAACAAAAAAAAATCTCTGAAACAATGGCGTGTTGCTTAGCTTCGTGCTGT
GCGTCGGCGACTTGTGGATTATGCTCGTCGGTAGCATCGGGGATATCGAGGAAATCAGCG
AGGATCGCTTATTGTGGTCTCTTCGGTGCCTCTCTTGTCGTCTCCTGGATTCTTCGTGAG
ACTGGTGCTCCGCTCCTCGAGAAACTCCCTTGTACGATTCTCTCATCCTTTTCTGAACTG
GGTTTGTGAAA

Last Updated on 06.12.2018 16:11