Genotyping and primer details


Genotyping and primer details for SALK_039574 - F2J7
Line ID SALK_039574
Locus F2J7 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8981160 of line SALK_039574 Chr1:8981160
   
Insertion allele amplicon R204 - BI08
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BI08: 5'-CCCAAGAAAAGACGTCTAAATGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8980575
   
Insertion allele amplicon LBb3 - BI09
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BI09: 5'-AACATGTCCAATTAAAACATGGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8981511
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11