Sequences from confirmation amplicon


Confirmation sequences for line SALK_039574 and hit F2J7 are shown.
Line ID SALK_039574
Locus F2J7 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8981160
Primers used for PCR BI08 - R204, show primer details
   
Sequence Name 66-K106742-SALK_039574C-R210
GenBank Accession LN485889 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K106742-SALK_039574C-R210
GTCATTTGTTGACATAACCACAACTTTTTGTTTCTCAATCATTTAATTACTTACGAGACA
TGCATTGGCAAGATATTTATATTCACTGGAGTAATGTTGAAATTTAGTAAATAAAATGTA
CATAAATGTATTTTCAGAATAACAAAAAGAAAACAAAATCAAAAGATCATTTGTTTATGA
ATTTATTATCGTCAACATATATTTAGTACTAGTTACTAAAACTAAACATAATTTTGATAA
AAATTAATCCTTATAACAAAAAACGTAAATAGCTAATGATTTTTCTGATTAAACATGAAA
ACATGGTTAAATGTTCTAAAACTAGTGTAATCCCTTACATTTAGTTGTTGAATATTGCTC
TCAATTCTCATGACTTGGAGCCCATGATGTACAAAAAGCCCATGCTCATTTAAAAGCCGA
ACGTTTGTTGATTTTCTAATGTTAGCGAACTTGAAACCTAAAACTTGTGGGTTATGGTAC
CACTTGAGATGACACATATAATATAAGCCCAACTCATGTTAGTTAAATTGTGACTCATTT
AGACGTCTTTTCTTGGG
   
Sequence Name 82-K106743-SALK_039574C-BI08
GenBank Accession LN486240 [GenBank]
Sequencing primer gene specific primer, BI08: 5'-CCCAAGAAAAGACGTCTAAATGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K106743-SALK_039574C-BI08
TGTGTCATCTCAAGTGGTACCATAACCCACAAGTTTTAGGCTTTCAAGTTCGCTAACATT
AGAAAATCAACAAACGTTCGGCTTCTAAATGAGCATGGGCTTTTTGTACATCATGGGCTC
CAAGTCATGAGAATTGAGAGCAATATTCAACAACTAAATGTAAGGGATTACACTAGTTTT
AGAACATTTAACCATGTTTTCATGTTTAATCAGAAAAATCATTAGCTATTTACGTTTTTT
GTTATAAGGATTAATTTTTATCAAAATTATGTTTAGTTTTAGTAACTAGTACTAAATATA
TGTTGACGATAATAAATTCATAAACAAATGATCTTTTGATTTTGTTTTCTTTTTGTTATT
CTGAAAATACATTTATGTACATTTTATTTACTAAATTTCAACATTACTCCAGTGAATATA
AATATCTTGCCAATGCATGTCTCGTAAGTAATTAAATGATTGAGAAACAAAAAGTTGTGG
TTATTGTCAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTGTTTTTTCTTTTC
Primers used for PCR BI09 - LBb3, show primer details
   
Sequence Name 27-K118709-SALK_039574C-BI09
GenBank Accession LN485073 [GenBank]
Sequencing primer gene specific primer, BI09: 5'-AACATGTCCAATTAAAACATGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118709-SALK_039574C-BI09
CTTAGCGGAGAACACGTCGATCGTTCAAGATTTTGTACGTTTCTTAAACTAAGTTTAAAA
ACTGTTTTTTCGTGTACGATTTTGGGGAAAAACACTGACCAAAATAATTAAGAACATGCA
CGGAATCTAGATTACGATTTTAGAACCATTGTTATGTTTAATAGAAATACATCTCGAAGT
TGATAATTATTTTTCTTATGCCTTAAGATTCATGCATCTTATTTATTTCACGAAGTATAG
TTGTGTTTAAAAAGAATCAATTAGATCTTAAAGGCTACACATGATGTATGTGTTATTAAG
TTGGCTGGCTGGTGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 02-K118708-SALK_039574C-R210
GenBank Accession LN484322 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118708-SALK_039574C-R210
CATTTGTTTACCCACAATATATCCTGCCACCAGCCAGCCAACTTAATAACACATACATCA
TGTGTAGCCTTTAAGATCTAATTGATTCTTTTTAACACAACTATACTTCGTGAAATAAAT
AAGATGCATGAATCTTAAGGCATAAGAAAAATAATTATCAACTTCGAGATGTATTTCTAT
TAAACATAACAATGGTTCTAAAATCGTAATCTAGATTCCGTGCATGTTCTTAATTATTTT
GGTCAGTGTTTTTCCCCAAAATCGTACACGAAAAAACAGTTTTTAAACTTAGTTTAAGAA
ACGTACAAAATCTTTGAACGATCGACGTTGTTCTCCGCTTAAGCATCTCAACCACATAAA
TAGATTTTCGAATCGCACCCATGTTTTAATTGGACATGT

Last Updated on 06.12.2018 16:11