Genotyping and primer details


Genotyping and primer details for SALK_040027 - At1g52370
Line ID SALK_040027
Locus At1g52370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19507155 of line SALK_040027 Chr1:19507155
   
Insertion allele amplicon LBb3 - 46sf
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46sf: 5'-AAGAAACATCGATTCAGAAGCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19507475
   
wt amplicon (over InsPos) 13ue - 46sf
wt amplicon start Chr1:19506675
wt amplicon end Chr1:19507475
wt amplicon size 801 bp
Locus specific primer 46sf: 5'-AAGAAACATCGATTCAGAAGCATT-3'
2nd locus specific primer 13ue: 5'-CAGTTTGTTTTGATGGTTAGGGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11