Genotyping and primer details


Genotyping and primer details for SALK_040039 - At1g52160
Line ID SALK_040039
Locus At1g52160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19424420 of line SALK_040039 Chr1:19424420
   
Insertion allele amplicon LBb3 - 54qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 54qz: 5'-GAGCAAACACGAGACAAAAATACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19423691
   
wt amplicon (over InsPos) 07rz - 54qz
wt amplicon start Chr1:19423691
wt amplicon end Chr1:19424635
wt amplicon size 945 bp
Locus specific primer 54qz: 5'-GAGCAAACACGAGACAAAAATACA-3'
2nd locus specific primer 07rz: 5'-ATAAAAACGCTAGAGAGAGGGGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11