Sequences from confirmation amplicon


Confirmation sequences for line SALK_040039 and hit At1g52160 are shown.
Line ID SALK_040039
Locus At1g52160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19424420
Primers used for PCR 54qz - LBb3, show primer details
   
Sequence Name 13-K115992-SALK_040039C-54qz
GenBank Accession LN484647 [GenBank]
Sequencing primer gene specific primer, 54qz: 5'-GAGCAAACACGAGACAAAAATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K115992-SALK_040039C-54qz
AATCATCAAAATTAAAACTCACCTCATCACACATACAAAGAGAAAACAGAGGAAACTTAC
CTTTGATAGTTTAATCTTATGTTCTGTACAAAAACGTTGCAAGCCCTAAAAAACACATTC
AATTCACAAGTAAGTGATCAAGTAACAAACTTTAAACTGCTGAAAAATCTGTAAGCGATT
TTAATTTACCTCTCCAGCATTAAAGATGAATCTTTGCTTATCAAAGAAGAGTAATACAGA
AGATGATGTATCCTGTGTATCCATTCCAGTTCCAAGTATCTGAAAGATCGGATTTTTAAG
TATTGTGAGAGATTAAGAATCCAAAATTGGAAAAAAAAACGAAGAAGTCTATGATGATGA
CCTGTGCGTAAGCAATAGTGTTAGTGGGATTAAGCTTTTGGGATTTGCGTTCTAGATTCC
TTTTTTTCTTATCGGTTATGTCAAATCCTTCAGCTCTTCGCTTATTGAACACGAATGAAT
TAGAAGAAGACTTGGAGCCATTAGTTGCTTCGTTGTTCTCCATTGAAGACGATGATGAAG
AAGAAGAGAAGAGGGTTGAGAGAGAGAAGGATCGTGGAGAAAAGGGTCTGAGAGAAAGAG
GGAAAGGAGAAGACTTTGAAGAGAGGAGACGAAGAAGGCGTAGGTTTTTGTGTAAATAGG
GCATTGAGTTTATCATCAAATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC
ACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAA
CAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGT
TTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
   
Sequence Name 04-K115991-SALK_040039C-R210
GenBank Accession LN484379 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115991-SALK_040039C-R210
CTCCTTGCCCTAATTACACGAAACCTACGCCTTCTTCGTCTCCTCTCGTTCTGTCTTCTC
CTTTCCCTCTCCCTCTCAAACCCTTTCCTCCACGATCCATCTATCTCTCAACCCTCTTCT
CTTCTTCTTCATCTTCGTCTTCAATGGAGAACAACGAATCCGCTAAGGGCTCCGATTCTT
CTTCTATCTTCATTCGTGTTCAAAAAGCAAAAAGCTGAATGATTTGCCATTACCGATAAA
AAAAAAAGGAATCTAGAACGCACATCCCAAAAGCTTAATCCCACTTACACTATTGCTTAC
GCACAGGTCAACATCATAGACTTCTTCGTTTTCTTTTCCAATTGTGGATTCTTCATCTCT
CACAATACTTAAACGTCCCATCTTTCAAATTCTTGGAACTGGAATGGATACTCACAAAAC
TCCACCTTCTGTATTACTCATCGTTGAAAAACAAAAATTCATCTCTGAGGCTGGAGAGGT
AAATTAAATTCGCTTCCTGATTTTCTCACCAAAATAAAGTTTGTTACTTGATCACTTACT
TGTGAATTGAGGGTGTTTTTTAGGGCTTGCACCGTTTTTGTACAAACCTTAAAATTAAA

Last Updated on 06.12.2018 16:11