Genotyping and primer details


Genotyping and primer details for SALK_040054c - At1g76970
Line ID SALK_040054c
Locus At1g76970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28923144 of line SALK_040054c Chr1:28923144
   
Insertion allele amplicon LBb3 - 33cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33cv: 5'-TAATTTCCTATTTTATGTGGGGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28922463
   
wt amplicon (over InsPos) 99dy - 33cv
wt amplicon start Chr1:28922463
wt amplicon end Chr1:28923530
wt amplicon size 1068 bp
Locus specific primer 33cv: 5'-TAATTTCCTATTTTATGTGGGGCA-3'
2nd locus specific primer 99dy: 5'-GGATTGGCATTAAACGATAATTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11