Genotyping and primer details


Genotyping and primer details for SALK_040252c - At1g63440
Line ID SALK_040252c
Locus At1g63440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23528028 of line SALK_040252c Chr1:23528028
   
Insertion allele amplicon R212 - 36tf
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 36tf: 5'-CAGTAAGCTCACCATCAATCTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23528399
   
Insertion allele amplicon LBb3 - CG25
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG25: 5'-TTTCAAAAAGTCTCATCATTTCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23527556
   
Insertion allele amplicon R212 - CG26
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer CG26: 5'-TCATCAGTAAGCTCACCATCAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23528403
   
wt amplicon (over InsPos) 36tf - CG25
wt amplicon start Chr1:23527556
wt amplicon end Chr1:23528399
wt amplicon size 844 bp
Locus specific primer CG25: 5'-TTTCAAAAAGTCTCATCATTTCCC-3'
2nd locus specific primer 36tf: 5'-CAGTAAGCTCACCATCAATCTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11