Genotyping and primer details


Genotyping and primer details for SALK_040287c - At3g02350
Line ID SALK_040287c
Locus At3g02350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:480253 of line SALK_040287c Chr3:480253
   
Insertion allele amplicon LBb3 - 43fp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 43fp: 5'-TGAGCTCCTTCTTTATTTCTCTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:479438
   
wt amplicon (over InsPos) 16go - 43fp
wt amplicon start Chr3:479438
wt amplicon end Chr3:480467
wt amplicon size 1030 bp
Locus specific primer 43fp: 5'-TGAGCTCCTTCTTTATTTCTCTGC-3'
2nd locus specific primer 16go: 5'-GATACTTGGGGTTCTTGAACTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11