Sequences from confirmation amplicon


Confirmation sequences for line SALK_040287c and hit At3g02350 are shown.
Line ID SALK_040287c
Locus At3g02350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:480253
Primers used for PCR 43fp - LBb3, show primer details
   
Sequence Name 78-K111509-SALK_040287C-43fp
GenBank Accession LN486187 [GenBank]
Sequencing primer gene specific primer, 43fp: 5'-TGAGCTCCTTCTTTATTTCTCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K111509-SALK_040287C-43fp
ATTAGAATGAATCGGTGGCAATTTCATTGAATCTGAGCTTTGATTTTTGCTGTCTGGTTT
TTAGACATTGCCGAGTATGGGCAACGCATATATGCAGAGGACGTTTTTGGCTTTGCAATC
GGATCCATTGAAAACTAGGTTGGATCTGATACACAAGCAAGCCATTGATCATTTGACACT
GGTGAATGCGTATGCTGCTTACGCTAGGAAGCTAAAGCTTGATGCTTCTAAGCAGCTTAA
GCTCTTCGAAGATTTGGCTATCAACTTCTCGGATTTGCAGTCGAAACCTGGTTTGAAATC
TGCTGTGTCTGATAATGGTAATGCTCTTGAGGAGGATTCGTTTAGGCAGCTTGAGAAAGA
AGTGAAGGATAAGGTGAAGACAGCGAGGATGATGATCGTTGAGTCTAAAGAGAGTTATGA
TACACAGCTTAAAATCCAGAAGTTGAAAGATACAATCTTTGCTGTCCAAGAACAGTTGAC
AAAGGCTAAGAAAAACGGTGCGGTTGCTAGCTTGATTTCAGCCAAGTCGGTTCCTAAAAG
TCTTCATTGTTTGGCCATGAGGCTTGTAGGAGAGAGGATCTCTAATCCTGAGAAGTACAA
GGATGCTCCACCTGACCCAGCCGCAGAGGATCCAACTCTTTACCACTATGCGATTTTCTC
TGATAATGTCATTGCTGTGTCTGTTGTGGTGAGATCGGTTGTGATGAACGCTGAGGAGCC
ATGGAAGCATGTCTTCCATGTGGTGACAGATCGGATGAATCTCCAGGATATATTGTGGTG
TAAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTT
   
Sequence Name 64-K111508-SALK_040287C-R210
GenBank Accession LN485846 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K111508-SALK_040287C-R210
GTCATTTGTTACCCACAATATATCCTGGAGATTCATCCGATCTGTCACCACATGGAAGAC
ATGCTTCCATGGCTCCTCAGCGTTCATCACAACCGATCTCACCACAACAGACACAGCAAT
GACATTATCAGAGAAAATCGCATAGTGGTAAAGAGTTGGATCCTCTGCGGCTGGGTCAGG
TGGAGCATCCTTGTACTTCTCAGGATTAGAGATCCTCTCTCCTACAAGCCTCATGGCCAA
ACAATGAAGACTTTTAGGAACCGACTTGGCTGAAATCAAGCTAGCAACCGCACCGTTTTT
CTTAGCCTTTGTCAACTGTTCTTGGACAGCAAAGATTGTATCTTTCAACTTCTGGATTTT
AAGCTGTGTATCATAACTCTCTTTAGACTCAACGATCATCATCCTCGCTGTCTTCACCTT
ATCCTTCACTTCTTTCTCAAGCTGCCTAAACGAATCCTCCTCAAGAGCATTACCATTATC
AGACACAGCAGATTTCAAACCAGGTTTCGACTGCAAATCCGAGAAGTTGATAGCCAAATC
TTCGAAGAGCTTAAGCTGCTTAGAAGCATCAAGCTTTAGCTTCCTAGCGTAAGCAGCATA
CGCATTCACCAGTGTCAAATGATCAATGGCTTGCTTGTGTATCAGATCCAACCTAGTTTT
CAATGGATCCGATTGCAAAGCCAAAAACGTCCTCTGCATATATGCGTTGCCCATACTCGG
CAATGTCTAANAACCAGACAGCANAAATCAAAGCTCAGATTCAATGAAATTGCCACCGAT
TCATTTCTAAATTTGTTCGAATTACAAAATCAATCANAAGCAGAGAAAT

Last Updated on 06.12.2018 16:11