Genotyping and primer details


Genotyping and primer details for SALK_040788 - At3g11240
Line ID SALK_040788
Locus At3g11240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3519787 of line SALK_040788 Chr3:3519787
   
Insertion allele amplicon R204 - BN23
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BN23: 5'-CTTGGAGCTGCTAAAAGAGAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3520222
   
Insertion allele amplicon LBb3 - BN24
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BN24: 5'-GTAGTTTCCAAGAGACAAGGATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3519395
   
wt amplicon (over InsPos) 10cj - BN23
wt amplicon start Chr3:3519338
wt amplicon end Chr3:3520222
wt amplicon size 885 bp
Locus specific primer BN23: 5'-CTTGGAGCTGCTAAAAGAGAAAAA-3'
2nd locus specific primer 10cj: 5'-AAGAGTAGAGCAATGAGCTTGGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11