Genotyping and primer details


Genotyping and primer details for SALK_040812c - At5g04410
Line ID SALK_040812c
Locus At5g04410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1246452 of line SALK_040812c Chr5:1246452
   
Insertion allele amplicon LBb3 - 40xh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 40xh: 5'-GCGATTTTATCTAATGGCATGTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1247052
   
Insertion allele amplicon LBb3 - CE65
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CE65: 5'-TTCTCTGTTTCCAGAGGAACAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1245409
   
wt amplicon (over InsPos) 29da - CE65
wt amplicon start Chr5:1245409
wt amplicon end Chr5:1246429
wt amplicon size 1021 bp
Locus specific primer CE65: 5'-TTCTCTGTTTCCAGAGGAACAAAT-3'
2nd locus specific primer 29da: 5'-AACTTTGATCCTATTACCGAGCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11