Sequences from confirmation amplicon


Confirmation sequences for line SALK_040812c and hit At5g04410 are shown.
Line ID SALK_040812c
Locus At5g04410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1246452
Primers used for PCR CE65 - LBb3, show primer details
   
Sequence Name 56-K107479-SALK_040812C-CE65
GenBank Accession LN485638 [GenBank]
Sequencing primer gene specific primer, CE65: 5'-TTCTCTGTTTCCAGAGGAACAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K107479-SALK_040812C-CE65
ATCTGGAATCTTCCACATAATGTGTTTGTAGCTGATACATGCTACAGTGACATTCCTATT
GATCATAACTATTTACCCGATGAGCCATTCATGGATCCTAATAACAATCTTCCACTCAAC
GATGGTCTGTACCTGGAAACGAATGATCTCAGCTGTGCTCAACAAGATGATTTTAACTTC
GAAGATTATCTCAGCTTCTTTGATGATGAGGGTTTGACTTTTGACGATTCTCTATTAATG
GGACCTGAAGATTTTCTTCCCAACCAAGAAGCCCTTGACCAGAAAGTAAGACCCGTTCTT
CCAACGTGCTTAGTTTATATATATGTGAAAAACTTCTGTTTAACTCACCTTTACATCAAC
AGCCTGCCCCTAAAGAATTGGAGAAGGAGGTCGCAGGAGGCAAAGAGGCAGTGGAGGAAA
AGGAAAGTGGCGAAGGATCTTCTTCAAAACAAGATACAGATTTCAAGGACTTTGATTCAG
GTAACACTCAGATGTTCATGGAATTCCTTTTAAAACAAGTCATATGATGACTGATTCTGA
CTCTTTTGAATCTAAATCATGTAGCTCCGAAGTACCCATTTCTCAAAAAGACGAGCCACA
TGCTTGGAGCCATTCCTACTCCATCTTCATTTGCTTCACAGTTCCAAACAAAGGACGCAA
TGCGTCTACACGCAGCACAATCTTCTGGTTCAGTTCACGTGACTGCAGGTATGATGAGAA
TATCAAACATGACTCTAGCAGCGGACAGCGGTATGGGCTGGTCATATGACAAGAACGGTA
ACCTCAACGTAGTCCTTTCATTCGGGGTAGTCCAACAGGATGATGCGATGACTGCCTCGG
GAAGCAAGACAGGAATTACGGCGACAAGAGCTATGTTAGTCTTCATGTGTTTATGGGTTC
T
   
Sequence Name 72-K107480-SALK_040812C-LBb3
GenBank Accession LN486049 [GenBank]
Sequencing primer T-DNA primer, LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K107480-SALK_040812C-LBb3
GGCACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAG
CTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCCAGTACATTAAAAACGTCCGCAA
TGTGTTATGAAGTTGTCTAATGTGTTATGCTATTGTGGTTTACCCCAGTTAGGGTACTAT
TTTGAAGCTAACAGAGAGTAGGAGAACCCATAAACACATGAAGACTAACATAGCTCTTGT
CGCCGTAATTCCTGTCTTGCTTCCCGAGGCAGTCATCGCATCATCCTGTTGGACTACCCC
GAATGAAAGGACTACGTTGAGGTTACCGTTCTTGTCATATGACCAGCCCATACCGCTGTC
CGCTGCTAGAGTCATGTTTGATATTCTCATCATACCTGCAGTCACGTGAACTGAACCAGA
AGATTGTGCTGCGTGTAGACGCATTGCGTCCTTTGTTTGGAACTGTGAAGCAAATGAAGA
TGGAGTAGGAATGGCTCCAAGCATGTGGCTCGTCTTTTTGAGAAATGGGTACTTCGGAGC
TACATGATTTAGATTCAAAAGAGTCAGAATCAGTCATCATATGACTTGTTTTAAAAGGAA
TTCCATGAACATCTGAGTGTTACCTGAATCAAAGTCCTTGAAATCTGTATCTTGTTTTGA
AGAAGATCCTTCGCCACTTTCCTTTTCCTCCACTGCCTCTTTGCCTCCTGCGACCTCCTT
CTCCAATTCTTTAGGGGCAGGCTGTTGATGTAAAGGTGAGTTAAACAGAAGTTTTTCACA
TATATATAAACTAAGCACGTTGGAAGAACGGGTCTTACTTTCTGGTCAAGGGCTTCTTGG
TTGGGAAGAAAATTTTAGGTCCCATTAAT
Primers used for PCR 40xh - LBb3, show primer details
   
Sequence Name 20-K119050-SALK_040812C-40xh
GenBank Accession LN484871 [GenBank]
Sequencing primer gene specific primer, 40xh: 5'-GCGATTTTATCTAATGGCATGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K119050-SALK_040812C-40xh
CAGGGCATCCATCGAAGCTGAAGTTGAGATGCTTAGAAGACAGAGGTCAGCGTCTGATGA
AGAAGAAGACGGGACTGTGCAAAGGCAAGGCTCTGCTGGTGTGTGGGGTTTGTTTGGAAG
GTAAATCCCAAACAGTTTGGCGACACAAATACCTATAAGCTCTGACGAAACAAACATTGG
TTTATAATTCCATTTTTTTGAGAATGAGAGATAGAGTGGGGGAAACATGTTCTTCTTTAT
TTGTTCTCAAAGATTCAAAATCAATTTGTTATGAATGCTTTTTTCCCTCATTTACTAGAG
ATTTTACAACGAAATATCATTTCACAATGTAACATGTTTAGACCAATGATTCCAACAATA
ACGGGATCAAATAATAATTGTCATAAGAAAGTTGTTTATGTTCTTATTTCTAGAAACTCA
AACTAGAAACCATATAGAGAATTCAAGCTACACCAAACCCTTCGAATAAAAATTTAAATA
TTGAGCAGCAAAGCACTGCCAAAGTTAAAGGCTATATGATTGGAGAGGTACAAACACCAG
TAAAATCCAAGCAATCGCATAAACACAATAACAGAACAAATTGTGGACGCATGAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CCGA
   
Sequence Name 04-K119049-SALK_040812C-R210
GenBank Accession LN484388 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K119049-SALK_040812C-R210
GTCCACATTTGTTCTGTTATTGTGTTTATGCGATTGCTTGGATTTTACTGGTGTTTGTAC
CTCTCCAATCATATAGCCTTTAACTTTGGCAGTGCTTTGCTGCTCAATATTTAAATTTTT
ATTCGAAGGGTTTGGTGTAGCTTGAATTCTCTATATGGTTTCTAGTTTGAGTTTCTAGAA
ATAAGAACATAAACAACTTTCTTATGACAATTATTATTTGATCCCGTTATTGTTGGAATC
ATTGGTCTAAACATGTTACATTGTGAAATGATATTTCGTTGTAAAATCTCTAGTAAATGA
GGGAAAAAAGCATTCATAACAAATTGATTTTGAATCTTTGAGAACAAATAAAGAAGAACA
TGTTTCCCCCACTCTATCTCTCATTCTCAAAAAAATGGAATTATAAACCAATGTTTGTTT
CGTCAGAGCTTATAGGTATTTGTGTCGCCAAACTGTTTGGGATTTACCTTCCAAACAAAC
CCCACACACCAGCAGAGCCTTGCCTTTGCACAGTCCCGTCTTCTTCTTCATCAGACGCTG
ACCTCTGTCTTCTAAGCATCTCAACTTCAGCTTCGATGGATTGCCCTGACTCCAGTGCTT
TCTGCAATTCTGCTATTTGTGCCTGAGACATGCCATTAGATAAAATCGCA

Last Updated on 06.12.2018 16:11