Genotyping and primer details


Genotyping and primer details for SALK_041400c - At4g20780
Line ID SALK_041400c
Locus At4g20780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11133363 of line SALK_041400c Chr4:11133363
   
Insertion allele amplicon LBb3 - 09rr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 09rr: 5'-TCTCCAACGAATCTTCGACTTATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11133798
   
wt amplicon (over InsPos) 10sx - 09rr
wt amplicon start Chr4:11132765
wt amplicon end Chr4:11133798
wt amplicon size 1034 bp
Locus specific primer 09rr: 5'-TCTCCAACGAATCTTCGACTTATT-3'
2nd locus specific primer 10sx: 5'-TATTCCACGACACAACATCTCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11