Sequences from confirmation amplicon


Confirmation sequences for line SALK_041400c and hit At4g20780 are shown.
Line ID SALK_041400c
Locus At4g20780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11133363
Primers used for PCR 09rr - LBb3, show primer details
   
Sequence Name 44-K116392-SALK_041400C-09rr
GenBank Accession LN485377 [GenBank]
Sequencing primer gene specific primer, 09rr: 5'-TCTCCAACGAATCTTCGACTTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K116392-SALK_041400C-09rr
CCAGCTCTACACGTCTTGGTCTCAACGCTGATCTCTCCGATCTCAAATCCACCGTTGAAT
CATACATCCAACCTGGTAACACTGGTCTCAATTTCGATGATTTCTCTTCTCTTCACAAAA
CACTCGACGATTCTTTCTTCGGTGGAGCTTGTGGAGGAGGAGAAAACGAAGATGATCCTT
CTTCCGCAGCTGAAAATGAATCGGATCTCGCCGAGGCGTTTAAGGTGTTTGATGAGAACG
GTGATGGATTCATCTCCGCTAGAGAACTGCAAACTGTTCTGAAGAAACTTGGTTTACCTG
AAGGTGGAGAAATGGAGAGAGTGGAGAAGATGATCGTTTCCGTTGACCGGAATCAAGATG
GTCGCGTTGACTTTTTTGAATTCAAGAACAAATTGACGCTTAGACAACTTAATAACACAT
TGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGC
TGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGC
CCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 19-K116391-SALK_041400C-R210
GenBank Accession LN484825 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116391-SALK_041400C-R210
AACGCGACCATCTTGATTCCGGTCAACGGAAACGATCATCTTCTCCACTCTCTCCATTTC
TCCACCTTCAGGTAAACCAAGTTTCTTCAGAACAGTTTGCAGTTCTCTAGCGGAGATGAA
TCCATCACCGTTCTCATCAAACACCTTAAACGCCTCGGCGAGATCCGATTCATTTTCAGC
TGCGGAAGAAGGATCATCTTCGTTTTCTCCTCCTCCACAAGCTCCACCGAAGAAAGAATC
GTCGAGTGTTTTGTGAAGAGAAGAGAAATCATCGAAATTGAGACCAGTGTTACCAGGTTG
GATGTATGATTCAACGGTGGATTTGAGATCGGAGAGATCAGCGTTGAGACCAAGACGTGT
TAGAGCTTGGCTTAGCTCTTCGACGGTTATGAATCCGTCGCCGTTTTTGTCGAATAAGTC
G

Last Updated on 06.12.2018 16:11