Genotyping and primer details


Genotyping and primer details for SALK_041504c - At2g26580
Line ID SALK_041504c
Locus At2g26580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11305096 of line SALK_041504c Chr2:11305096
   
Insertion allele amplicon LBb3 - 25ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25ux: 5'-AAAACTTACATTCTTGGCAGCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11304542
   
wt amplicon (over InsPos) 50vl - 25ux
wt amplicon start Chr2:11304542
wt amplicon end Chr2:11305616
wt amplicon size 1075 bp
Locus specific primer 25ux: 5'-AAAACTTACATTCTTGGCAGCAGT-3'
2nd locus specific primer 50vl: 5'-CCTCAATTGTGAAGGGTTTATTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11