Genotyping and primer details


Genotyping and primer details for SALK_042206c - At4g33700
Line ID SALK_042206c
Locus At4g33700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16176603 of line SALK_042206c Chr4:16176603
   
Insertion allele amplicon LBb3 - 66kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 66kh: 5'-ACTTGATTCTTGAGAAAGGGCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16177482
   
wt amplicon (over InsPos) 01mu - 66kh
wt amplicon start Chr4:16176506
wt amplicon end Chr4:16177482
wt amplicon size 977 bp
Locus specific primer 66kh: 5'-ACTTGATTCTTGAGAAAGGGCATA-3'
2nd locus specific primer 01mu: 5'-TTTCGACATTCATAACCAGTTCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11