Genotyping and primer details


Genotyping and primer details for SALK_042239 - At1g11990
Line ID SALK_042239
Locus At1g11990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4048512 of line SALK_042239 Chr1:4048512
   
Insertion allele amplicon LBb3 - 17hd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17hd: 5'-TTGGTAGCTCCTTTACTATCCGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4047676
   
wt amplicon (over InsPos) 13il - 17hd
wt amplicon start Chr1:4047676
wt amplicon end Chr1:4048734
wt amplicon size 1059 bp
Locus specific primer 17hd: 5'-TTGGTAGCTCCTTTACTATCCGAA-3'
2nd locus specific primer 13il: 5'-ACAGGTCCAATGATCGTAATCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11