Genotyping and primer details


Genotyping and primer details for SALK_042314c - At5g66140
Line ID SALK_042314c
Locus At5g66140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26437465 of line SALK_042314c Chr5:26437465
   
Insertion allele amplicon LBb3 - 55ew
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 55ew: 5'-CTGGCCAAGAAACTATTAAACTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26437748
   
wt amplicon (over InsPos) 29gc - 55ew
wt amplicon start Chr5:26436873
wt amplicon end Chr5:26437748
wt amplicon size 876 bp
Locus specific primer 55ew: 5'-CTGGCCAAGAAACTATTAAACTCG-3'
2nd locus specific primer 29gc: 5'-AAATTTGATGTGTGAATGCAGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11