Sequences from confirmation amplicon


Confirmation sequences for line SALK_042314c and hit At5g66140 are shown.
Line ID SALK_042314c
Locus At5g66140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26437465
Primers used for PCR 55ew - LBb3, show primer details
   
Sequence Name 61-K111283-SALK_042314C-55ew
GenBank Accession LN485763 [GenBank]
Sequencing primer gene specific primer, 55ew: 5'-CTGGCCAAGAAACTATTAAACTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K111283-SALK_042314C-55ew
TCTCGTCCAGCTGATATTTTGTTATTTCAGGATTTTTTGTCCTTTGAGATCATAGCTGTT
TTGATATCGGCTTTGCATTGCAGGTAGTTGAGAGTGGCGGAAAGAACATTGAGGTTGCCG
TAATGACACGGGAGGAAACTGGGCTGCGCCAGCTAGAAGAAGCTGAAATTGATGCAATCG
TGGCCAAGATCGAAGCTGAAAAGGCGGCCGCAGAAGCAGCCAAGAAAGGCCCTCCAAAGG
AAACTTGATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCG
GTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCNNGC
AAAA
   
Sequence Name 45-K111282-SALK_042314C-R210
GenBank Accession LN485393 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K111282-SALK_042314C-R210
GAGGCTTTCTTGGCTGCTTCTGCGGCCGCCTTTTCAGCTTCGATCTTGGCCACGATTGCA
TCAATTTCAGCTTCTTCTAGCTGGCGCAGCCCAGTTTCCTCCCGTGTCATTACGGCAACC
TCAATGTTCTTTCCGCCACTCTCAACTACCTGCAATGCAAAGCCGATATCAAAACAGCTA
TGATCTCAAAGGACAAAAAATCCTGAAATAACAAAATATCAGCTAGGACGGAGAGGGCAC
CAACCTCAAGCAGAGCACGGATAGCGAGTTTAATAGTTTCTTGGCCA

Last Updated on 06.12.2018 16:11